Figure 6. WT1-AS can inhibit the miR-375/SIX4 axis to suppress OSI and apoptosis by downregulating the transcription factor WT1. (A) The expression of SIX4 detected by qRT-PCR in cells transfected with SIX4-1 and SIX4-2 to screen a siRNA sequence. (B) The expression of WT1-AS, WT1, miR-375 and SIX4 detected by qRT-PCR. (C) The expression of p-Tau and total Tau detected by western blot. (D) Detection of mitochondrial membrane potential by JC-1 staining. (E) Detection of ATP content. (F) Detection of ROS content. (G) Detection of MDA content, SOD and GSH-Px activities. (H) Detection of LDH activity. (I) Detection of apoptosis by flow cytometry. *P<0.05; the experimental results are expressed as the mean ± standard deviation. Differences among multiple groups were analyzed using one-way ANOVA followed by Tukey’s post hoc test. The experiment was repeated three times.