Research Paper Advance Articles pp 23974—23995

Effect of lncRNA WT1-AS regulating WT1 on oxidative stress injury and apoptosis of neurons in Alzheimer's disease via inhibition of the miR-375/SIX4 axis

Figure 7. WT1-AS inhibited OSI and apoptosis of neurons in AD in vivo. (A) Detection of the learning and memory abilities of mice by using the Morris water maze test. (B) The expression of WT1-AS, WT1, miR-375 and SIX4 detected by qRT-PCR in brain tissues of mice. (C) The expression of p-Tau and total Tau detected by western blot. (D) Detection of ROS content. (E) Detection of MDA content, SOD and GSH-Px activities. (F) Detection of LDH activity. (G) Detection of apoptosis by TUNEL staining (400x). *P<0.05; the experimental results were expressed as the mean ± standard deviation. Differences among multiple groups were analyzed using one-way ANOVA followed by Tukey’s post hoc test, n = 10 mice.