Figure 1. Oleate/palmitate induces an in vitro model NAFLD. HepG2 cells were incubated with oleate/palmitate (2:1 molar ratio) for 0, 12, 24 and 36 h. (A) CCK-8 assays were used to measure cell viability. (B, C) Apoptotic cells were detected with Annexin V and PI double staining. (D, E) Lipid deposition in HepG2 cells detected with Oil red O staining. Magnification: 200x. *P<0.05, **P<0.01 vs. control group.