Research Paper Advance Articles pp 23996—24008

Hsa_circ_0048179 attenuates free fatty acid-induced steatosis via hsa_circ_0048179/miR-188-3p/GPX4 signaling

Figure 5. Downregulation of miR-188-3p attenuates oleate/palmitate-induced lipid accumulation in HepG2 cells. (A) HepG2 cells were transfected with miR-188-3p antagomir for 48 h and then exposed to oleate/palmitate (2:1 molar ratio) for another 24 h. CCK-8 assays were used to measure cell viability. **P<0.01 (B) Apoptotic cells were detected with Annexin V and PI double staining. (C) ROS generation were detected as DCF fluorescence with flow cytometry. (D) Levels of GPX4 expression in HepG2 cells were detected with western blotting. (E) Relative GPX4 expression in HepG2 cells was quantified by normalization to GAPDH. **P<0.01.