Research Paper Advance Articles pp 23996—24008

Hsa_circ_0048179 attenuates free fatty acid-induced steatosis via hsa_circ_0048179/miR-188-3p/GPX4 signaling

Figure 6. Overexpression of hsa_circ_0048179 attenuates oleate/palmitate-induced lipid accumulation in HepG2 cells by sponging miR-188-3p. HepG2 cells were transfected with miR-188-3p agomir or co-transfected with hsa_circ_0048179 and miR-188-3p agomir for 48 h, and then exposed to oleate/palmitate (2:1 molar ratio) for another 24 h. (A) CCK-8 assays were used to measure cell viability. **P<0.01 (B, C) Apoptotic cells were detected with Annexin V and PI double staining. **P<0.01 (D) ROS generation were detected as DCF fluorescence with flow cytometry. (E, F) JC-1 staining was used to determine mitochondrial membrane depolarization. **P<0.01.