Figure 3. PM-derived EVs reduce PLZF expression in HMrSV5 cells and promote their invasive ability. (A) CCK-8 assay indicated that EVs isolated from GC patients with PM significantly increased the viability of HMrSV5 cells compared to that of without PM. (n=3, one way ANOVA) (B) Trans-well assay illustrating invasion of HMrSV5 cells incubated with EVs isolated from peritoneal fluid of GC patients with and without PM. (C) For migration assays, more adhesive HMrSV5 cells were found in those pretreated by EVs isolated from peritoneal fluid in GC patients with PM compared those without PM. (D) Immunofluorescence and (E) western blotting showing that pretreatment with EVs isolated from patients with PM reduces the PLZF expression in HMrSV5 cells. (n=3, Student’s t-test for B, C, D, E).