Figure 2. NEAT1 silencing induces de-polymerization of microtubules (MTs). (A) Immunofluorescence staining of Ace-tubulin (red) in shNEAT1v2 cells and shCtrl cells. Scale bars, 20μm. (B) Western blot analysis for acetylated tubulin expression in shNEAT1v2 cells and shCtrl cells as well as neurons. (C) Immunofluorescence analysis of α-tubulin (red) in shNEAT1v2 cells and shCtrl cells in microscope high power fields. Scale bars, 50μm. (D) NEAT1 siRNA and control siRNA transiently transfected SH-SY5Y cells were treated with 0.5μM taxol for 72 hours. And then immunostained with α-tubulin (red). DAPI (blue) was used to stain the nuclei. Scale bars, 100μm. Image J software was used to analyze the cell dendritic length (mean ± s.d, *P < 0.05, **P < 0.01, ***P < 0.001, Student 2-tailed t test).