Figure 2. The expression and localization of TREM2 and FoxO3a in the hippocampus in 5xFAD mice. (A, B) Western blot bands and its quantificational analysis of TREM2 (n=6), respectively. (F, G) Western blot bands and its quantificational analysis of FoxO3a (n=4), respectively. In which, p-FoxO3a was normalized to total FoxO3a expression and total FoxO3a was normalized to GAPDH. (C, H) The mRNA levels of TREM2 (n=6) and FoxO3a (n=4) were measured by RT-PCR in the hippocampus. (D, I) Brain sections were doubly stained with Iba-1 (green) Ab for microglia and TREM2 Ab (red) or p-FoxO3a Ab (red) in the hippocampus from 5xFAD and WT mice at 7 months old. (E, J) Quantificational analysis of the expression of TREM2 and p-FoxO3a, respectively, which were matched with Fig D and Fig I (n=5), respectively. Original magnification, 20x; Scale bar=100 μm. * P<0.05, ** P<0.01, *** P<0.001, vs WT group. Bars were represented as mean ± SEM.