Research Paper Volume 12, Issue 20 pp 20862—20879

TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer’s disease mice

Figure 3. TREM2 defect aggravated cognitive and memory function in 5xFAD mice. (A) Schematic diagram of mouse brain hippocampal injection of AAV-TREM2 or control AAV. (B, C) western blot bands (B) and its quantificational analysis (C) of TREM2 in each groups (n=4). (D) Quantificational analysis of TREM2 mRNA by RT-PCR. (E, F) Total distance and center times were examined in open field test. (G, H) Swim speed and escape latency were recorded in training days. (I, J) Time percentage in target quadrant per group (I) and cross times (J) were recorded during the probe test. (K) Time to visible platform was examined in cued test. (L) Representative runs of three groups of mice in the probe test in MWM. Q1 was the target quadrant where the platform was located in the training days. Statistical difference was performed by two-way ANOVA and one-way ANOVA. * P<0.05, ** P<0.01, *** P<0.001, vs 5xFAD group; # P<0.05, ## P<0.01, ### P<0.001, vs 5xFAD-NC group. Results were presented as mean±SEM, n=12 per group.