Research Paper Volume 12, Issue 20 pp 20862—20879

TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer’s disease mice


Figure 5. TREM2 downregulation inactivates the PI3K/AKT/FoxO3a pathway and increases the inflammatory cytokine levels. (A, B) Western blot bands (A) and quantificational analysis (B) of p-PI3K, p-AKT, p-FoxO3, IL-6, IL-1β and TNF-α in all groups (n=4). (C) The mRNA levels of proinflammatory cytokines (IL-1β, IL-6, TNF-α) were detected by qPCR (n=4 per group). *P<0.05, **P<0.01, ***P<0.001, vs 5xFAD group; # P<0.05, ## P<0.01, ### P<0.001 vs 5xFAD-NC group. Data were presented as mean ± SEM.