Research Paper Volume 12, Issue 20 pp 20862—20879

TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer’s disease mice

Figure 9. Experimental design and groups. TREM2, Triggering receptor expressed on myeloid cells 2; 5xFAD group was injected with PBS; 5xFAD-NC group was injected with scramble sgRNA (CRISPR/Cas9 on AAV); 5xFAD-AAV-TREM2 group was injected with TREM2 specific sgRNA (CRISPR/Cas9 on AAV). Sham, treated with PBS only; LPS, treated with lipopolysaccharide; LPS+NC, treated with LPS and negative plasmid; LPS+OE, treated with LPS and TREM2 plasmid; NfL, Neurofilament light chain; IF, immunofluorescence; LY294002, PI3K inhibitor; qPCR, Real-time PCR; WB, Western blot.