Figure 6. The intrinsic apoptotic pathway was activated after administration of H2O2-induced oxidative stress. (A) The expression of proteins of intrinsic apoptotic pathway. Quantified protein expression ratio of (B) acetylated p53 (K382) and (C) caspase-9 (Cas-9) were significantly increased after the addition of H2O2, indicating the activation of an intrinsic pathway. In contrast, the quantified protein expression ratio of (D) caspase-8 (Cas-8) did not vary significantly, implying that the extrinsic pathway was not involved. The increases in (E) caspases-3 (Cas-3) and (F) cleaved poly ADP-ribose polymerase (c-PARP) indicated the activation of apoptosis. (n = 6; * p < 0.05 compared to the control group (0’); the value was generated by one-way ANOVA and post-hoc Tukey’s test.).