Research Paper Volume 12, Issue 22 pp 23326—23336

The DLG1-AS1/miR-497/YAP1 axis regulates papillary thyroid cancer progression

Figure 6. Knockdown of DLG1-AS1 suppressed tumor growth in PTC xenograft model. (A) Tumor volumes were examined every 7 days until the mice were sacrificed. (B) Tumor image was captured at the end of experiments. (C) Tumor weight was measured at the end of experiments. (D) The expression of Ki-67 was determined in xenograft tumor by immunohistochemistry (IHC). (E) The expression of DLG1-AS1 was determined in xenograft tumor by quantitative real-time polymerase chain reaction (qRT-PCR). (F)The expression of miR-497 was examined in xenograft tumor by qRT-PCR. (G, H) The mRNA and protein expression of YAP1 measured in xenograft tumors. All experiments were performed in triplicate and data are expressed as the mean ± standard deviation (SD) (*P < 0.05, **P < 0.01).