Research Paper Advance Articles pp 24208—24218

SRT2183 impairs ovarian cancer by facilitating autophagy

Figure 4. SRT2183 inhibits AKT/mTOR/p70S6K pathway and actives p38 MAPK signaling pathway. OVCAR-3 and A2780 cells were treated by 1 μM SRT218 for 12 h, 24 h, or 48 h. (A) The activities of AKT/mTOR/p70S6K pathway and (B) MAPK pathway were evaluated by western blot. (C) In order to evaluate if SRT2183 induce the autophagy by p38 signaling pathway, the cells were treated by SB203580, a traditional inhibitor of p38 MAPK signaling pathway. All experiments were repeated for three times.