Figure 1. Increased TNFSF14 expression in mice after UUO surgery. (A) TNFSF14 expression in kidney tissues at the indicated time points in Tnfsf14+/+ mice after UUO surgery was assessed by qRT-PCR. GAPDH was used as the internal control. (B) The expression of TNFSF14 in the kidney tissues of Tnfsf14+/+ mice after UUO for 7 days was detected by immunohistochemistry (upper lane, original magnification ×200; lower lane, original magnification ×400). (C) TNFSF14 levels in serum in Tnfsf14+/+ mice at the indicated time points after UUO surgery were measured by ELISA. The data were representative of the results of three independent experiments. All values are represented as mean ± SEM. Sham group was used as the UUO control. n = 5 per group. *P < 0.01, **P < 0.01 and ***P < 0.001.