Figure 3. Tnfsf14 deficiency ameliorates UUO-induced renal fibrosis. Kidney tissues from Tnfsf14+/+ and Tnfsf14−/− mice were collected at 7 days after UUO surgery. Sham group was used as the control of UUO. (A) Sirius Red and Masson staining of kidney tissues sections. Original magnification ×400. (B) α-SMA expression in kidney tissues was measured by immunohistochemistry. Original magnification ×200. (C) Western blot analyses of renal fibronectin and α-SMA expression in kidney tissues. Representative western blot (Left) and quantitative data (Right) are presented. (D) The mRNA levels of pro-fibrotic mediators Cola1, Vim, and TGF-β1 were measured by qRT-PCR. The data were representative of the results of three independent experiments. All values are represented as mean ± SEM. n = 5 per group. **P < 0.01 and ***P < 0.001.