Figure 5. Reduced inflammatory responses in Tnfsf14-deficient mice after UUO. Kidney tissues of Tnfsf14+/+ and Tnfsf14−/− mice were collected after UUO surgery for 7 days. (A) Infiltration of neutrophils (Ly-6G+, white arrows), macrophages (F4/80+, white arrows), and T lymphocytes (CD3+, white arrows) in kidney tissues was assessed by immunofluorescence. Original magnification ×400. (B) The mRNA levels of inflammatory cytokines TNF-α, IL-6, IL-1β, and IL-10 in kidney tissues were measured by qRT-PCR. Sham group was used as the control of UUO. The data were representative of the results of three independent experiments. Values are represented as means ± SEM. n = 5 per group. **P < 0.01 and ***P < 0.001.