Research Paper Volume 12, Issue 24 pp 25469—25486

Tumor necrosis factor superfamily 14 is critical for the development of renal fibrosis

Figure 7. Tnfsf14 deficiency leads to a remarkable reduction in UUO-induced Sphk1 expression in kidney tissues of mice. After UUO surgery for 7 days, kidney tissues from Tnfsf14+/+ and Tnfsf14−/− mice were collected. (A) The expression of Sphk1 in kidney tissues was measured by immunohistochemistry (upper lane, original magnification ×200; lower lane, original magnification ×400). (B) The expression of Sphk1 in kidney tissues was measured by western blot. Representative western blot (Left) and quantitative data (Right) are presented. (C) The expression of Sphk1 mRNA in kidney tissues was measured by qRT-PCR. Sham group was used as the control of UUO. (D) Primary cultured mTECs were stimulated with rmTNFSF14 (100 ng/mL) for 24 h. Medium was used as the negative control. Western blot analyses of Sphk1 protein of mTECs. Representative western blot (Left) and quantitative data (Right) are presented. The data were representative of the results of three independent experiments. All values are represented as means ± SEM. n = 5 per group. **P < 0.01 and ***P < 0.001.