Research Paper Advance Articles pp 24242—24254

TNFAIP3 ameliorates the degeneration of inflammatory human nucleus pulposus cells by inhibiting mTOR signaling and promoting autophagy

Figure 6. TNFAIP3 inhibits the inflammatory response and promotes the synthesis of the extracellular matrix. (A, C) TNFAIP3 enhanced matrix production by the inhibition of mTOR signaling. Matrix production was measured by Western blot for the levels of COL2A1 and Aggrecan (A) With their quantification normalized to GAPDH (B, C). (D, E) TNFAIP3 decreased the protein level of inflammatory cytokines IL-1β (D) and TNF-α (E) in the supernatants of the NPCs culture as quantified by ELISA. Data are represented by the mean ± standard deviation of 3 independent replicates. *** P<0.01, ** P<0.05, * P>0.05 by the Student t-test. Torin1 treated cells at 20 nM for 24 h.