Figure 5. AFM tapping mode images of brain-derived supported lipid bilayers. (A) Supported bilayer made from naked mole-rat brain-derived lipids in PBS. More than 80% of the membrane is made up of phase separated islands of ~1.5 nm in height relative to the surrounding liquid disordered phase. (B, C) Supported bilayer made of naked mole-rat brain lipids showing a two-tier raft structure, the horizontal blue line corresponding to the height profile in C, where the two-tier raft structure is evident from the peaks having a shoulder, the first at ~0.75 nm, the second at ~1.25 nm, indicated by arrows in the figure. (D) Supported lipid bilayer made from mouse brain derived lipids. In contrast to the naked mole-rat bilayers there is little evidence of phase separation.