Figure 4. TNFα blockade reduced inflammation. (A) Representative images of muscle sections analyzed at 22 or 28 months of age in control (saline) and Etanercept-treated mice. Scale bar=50 μm. Arrows indicate perivascular inflamed areas. (B) Representative images of muscle CD68- and CD3-positive cells (both in red) in control and Etanercept-treated muscle sections at 22 and 28 months of age. Laminin staining (green) identifies fibers while cell nuclei are blue. Scale bar=50 μm (magnification of 16x acquisition). (C) Inflammation score and CD68+ and CD3+ cell number evaluated in 12 months old mice and in control and Etanercept-treated mice at 22 and 28 months of age.