Research Paper Volume 13, Issue 2 pp 2118—2134

IGF2 improves the developmental competency and meiotic structure of oocytes from aged mice

IGF2 improves the mitochondrial functional activity of oocytes from aged mice. (A) Mitochondria were stained with mitotracker Green FM (green). Scale bar = 20 μm. (B) Quantification of mitochondrial distribution signals in control oocytes (n = 26) and IGF2-treated oocytes (n = 25). A Student’s t-test (two-tailed). *p C) JC-1 staining showing the mitochondrial membrane potential (MMP) in control and IGF2-treated oocytes. (D) Quantification of the red/green fluorescence intensity ratio in control oocytes (n = 40) and IGF2-treated oocytes (n = 35). A Student’s t-test (two-tailed). *p E) HPG Fluorescent staining showing total protein synthesis in MII-stage oocytes with or without IGF2-treatment. Oocytes were incubated in M16 medium with 50 μM HPG for 1 h prior to staining. Scale bar = 30 μm. (F) Quantification of HPG signal intensity in control (n = 28) and IGF2-treated (n = 29) oocytes. *p t-test (two-tailed). Error bars indicate the SEM.

Figure 4. IGF2 improves the mitochondrial functional activity of oocytes from aged mice. (A) Mitochondria were stained with mitotracker Green FM (green). Scale bar = 20 μm. (B) Quantification of mitochondrial distribution signals in control oocytes (n = 26) and IGF2-treated oocytes (n = 25). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (C) JC-1 staining showing the mitochondrial membrane potential (MMP) in control and IGF2-treated oocytes. (D) Quantification of the red/green fluorescence intensity ratio in control oocytes (n = 40) and IGF2-treated oocytes (n = 35). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (E) HPG Fluorescent staining showing total protein synthesis in MII-stage oocytes with or without IGF2-treatment. Oocytes were incubated in M16 medium with 50 μM HPG for 1 h prior to staining. Scale bar = 30 μm. (F) Quantification of HPG signal intensity in control (n = 28) and IGF2-treated (n = 29) oocytes. *p < 0.05. A Student’s t-test (two-tailed). Error bars indicate the SEM.