Research Paper Volume 12, Issue 22 pp 22495—22508

Cell differentiation and aging accompanied by depletion of the ACE2 protein

Figure 4. Distribution of ACE2 (green) in wt and HDAC1-depleted mES cells, non-differentiated and differentiated into cardiomyocytes. Immunofluorescence analyses were performed in (A) HDAC1 wt mESCs, (B) HDAC1 dn mESCs, (C) α-actinin (red)-positive cardiomyocytes generated from HDAC1 wt mESCs, and (D) α-actinin (red)-positive cardiomyocytes generated from HDAC1 dn mESCs. DAPI (blue) was used as a counterstain. (E) The nucleo/cytoplasmic ratio of the level of ACE2 is shown for HDAC1 wt mESCs. (F) The nucleo/cytoplasmic ratio of the level of ACE2 is shown for HDAC1 dn mESCs.