Figure 4. Effects of novel C. sativa extracts on the levels of ACE2 in the EpiOral tissue models upon induction of inflammation by treatment with TNFα/IFNγ. (A, B) Total RNA was isolated from 3D EpiOral tissues and subjected to RNA-Seq analysis as described in the “Methods”. The levels of ACE2 gene expression is presented as an average (with SD) from two samples. * - Statistically significant, ANOVA-like analysis and pair-wise comparison, as per Materials and Methods. (C) Two tissue samples were used per treatment group. Whole lysates prepared from EpiOral tissues were subjected to Western blotting using antibody against ACE2 as described in the “Methods”. The relative densitometry is presented as an average (with SD) from three technical repeat measurements. * - p<0.05, Student’s t-test.