Research Paper Volume 13, Issue 2 pp 2198—2211

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

Effects of circ

Figure 2. Effects of circ_0005198 expression on the progression of TMZ-resistant glioma cells. (A) qRT- PCR analysis of circ_0005198 expression in transfected U138/TMZ and LN18/TMZ cells. (B) The viability of U138/TMZ and LN18/TMZ cells transfected with si-circ_0005198-2 were detected by CCK-8 assay. (C) The proliferative rate of transfected U138/TMZ and LN18/TMZ cells was determined by colony formation assay. (D) The apoptotic rate of cells was determined using flow cytometry after circ_0005198 knockdown in U138/TMZ and LN18/TMZ cells. (E) CCK-8 assay was performed to measure the TMZ resistance of U138/TMZ and LN18/ TMZ cells. *P <0.05, **P < 0.01, *‘*P < 0.001.