Research Paper Volume 13, Issue 2 pp 2198—2211

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

Circ 0005198 targeted miR-198. (A) qRT-PCR showed the enrichment of circ

Figure 3. Circ 0005198 targeted miR-198. (A) qRT-PCR showed the enrichment of circ_0005198 in cytoplasmic. (B) The predicted binding sites between circ_0005198 and miR-198. (C) MiR-198 expression in U138/TMZ and LN18/TMZ cells was detected by qRT-PCR after transfection with miR-138 mimic. (D) Luciferase reporter assay was performed for the confirmation of direct binding relationship between circ_0005198 and miR-198 with luciferase reporter plasmids of wild-type and mutant circ_0005198. (E) qRT-PCR was performed to detect the effect of circ_0005198 on miR-198 expression. (F) Enrichment of circ_0005198 and miR-198 using Ago2 antibody in U138/TMZ and LN18/TMZ cells was tested by RIP assay. **P < 0.01, ***P < 0.001.