Research Paper Volume 13, Issue 2 pp 2198—2211

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

TRIM14 was up-expressed in glioma and its silencing inhibited the progression of TMZ-resistant glioma cells. (A, B) Analysis of the relative expression of TRIM14 in glioma tissues, glioma cells (U138 and LN18) and TMZ-resistant glioma cells (U138/TMZ and LN18/TMZ). (C) qRT-PCR was used to measure the expression of TRIM14 in U138/TMZ and LN18/TMZ cells transfected with si-TRIM14. (D, E) The viability was detected by CCK-8 and colony formation assays in transfected cells. (F) Apoptotic rate was detected by flow cytometry. (G) The TMZ resistance of cells was measured by CCK-8 assay. *P

Figure 7. TRIM14 was up-expressed in glioma and its silencing inhibited the progression of TMZ-resistant glioma cells. (A, B) Analysis of the relative expression of TRIM14 in glioma tissues, glioma cells (U138 and LN18) and TMZ-resistant glioma cells (U138/TMZ and LN18/TMZ). (C) qRT-PCR was used to measure the expression of TRIM14 in U138/TMZ and LN18/TMZ cells transfected with si-TRIM14. (D, E) The viability was detected by CCK-8 and colony formation assays in transfected cells. (F) Apoptotic rate was detected by flow cytometry. (G) The TMZ resistance of cells was measured by CCK-8 assay. *P < 0.05, **P < 0.01, ***P < 0.001.