Figure 1. ISO inhibits HCC cell growth. (A) RTCA of proliferation of HCC cells treated with different concentration of CCE. (B) CellTiter-Blue viability assay results from HepG2 and Huh7 cells treated with 100-500 μM ISO or 0.2% dimethyl sulfoxide (DMSO; vehicle) for 24, 48, and 72 h. (C) Representative images of HepG2 and Huh7 cell colonies. Cells were treated with ISO and allowed to grow for 2 weeks before quantification of cell colonies stained with crystal violet. (D) Quantification of crystal violet-stained cell colonies. Values represent mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001.