Figure 4. Hsa_circ_0079480 served as sponge for miR-654-3p. (A) Two potential target miRNAs (miR-346 and miR-654-3p) of hsa_circ_0079480 were predicted by circBank and CircInteractome. (B) Pull-down assay explored the relative expression of two miRNAs in AML cells lysates. (D) Schematic of the predicted binding site in hsa_circ_0079480. (C, E) The correlation among hsa_circ_0079480 and miR-654-3p was explored through luciferase reporter and RIP assays. (F) Hsa_circ_0079480 knockdown increased miR-654-3p levels in AML cells. *P<0.05.