Research Paper Advance Articles pp 24345—24356

Implication of integrins α3β1 and α5β1 in invasion and anoikis of SK-Mel-147 human melanoma cells: non-canonical functions of protein kinase Akt

Figure 4. Effect of Akt isoform inhibitors on in vitro invasion of SK-Mel-147 cells. Cells transduced with an appropriate shRNA were incubated 48 hours with 3 μM Akt1-specific inhibitor XXIII or 5 μM Akt2-specific inhibitor XII, after which the in vitro invasion was determined as described in Materials and Methods. The invasion of cells transduced with a control vector and not treated with inhibitors was taken as 100%. The results of 3 independent experiments (M ± SEM) are presented.