Research Paper Volume 13, Issue 2 pp 2231—2250

Matrix stiffness regulates myocardial differentiation of human umbilical cord mesenchymal stem cells

Expression of myocardial markers on protein level. (A) The photograph of Western Blot detected the expression of Nkx2.5, GATA4, Mesp1, cTnT, cTnI and α-actin on protein level. (B) Graph of the Western Blot. *PPP

Figure 7. Expression of myocardial markers on protein level. (A) The photograph of Western Blot detected the expression of Nkx2.5, GATA4, Mesp1, cTnT, cTnI and α-actin on protein level. (B) Graph of the Western Blot. *P<0.05, **P<0.01, ***P<0.001. Nkx2.5, GATA4, Mesp1, and cTnI were all highly expressed on 13-16 kPa matrix, compared with 62-68kpa and control group. n=3.