Figure 2. ARRB2 inhibits hepatocyte apoptosis in hepatic ischemia-reperfusion injury. (A) and (B), TUNEL staining of the liver tissue and quantitative analysis of TUNEL positive cells (green) in Sham, WT + IR and KO + IR groups. (C) and (D), Cleaved Caspase-3 protein level in liver tissue detected by Western Blotting. β-actin was used as an internal control. (E) and (F), The Immunohistochemical staining of Cleaved Caspase-3 in liver tissues. The data are presented as the Mean ± SD, n = 6. *P< 0.01, compared to Sham group, #P < 0.01, compared to WT + IR group by Student's-t test.