Figure 5. PX866 inhibits PI3K/Akt signaling activation. (A) PI3K, p-PI3K, AKT, p-AKT protein levels at 6 h after reperfusion detected by Western Blotting in Sham, WT + IR, KO + IR, and WT + IR + PX866 groups. (B) Quantitative analysis of p-PI3K/PI3K levels and p-Akt/Akt levels. (C) and (D), Immunohistochemical staining of p-Akt in liver tissues (×200). The data are presented as the Mean ± SD, n = 6. *P<0.01, compared to Sham group, #P< 0.01, compared to WT + IR group by Student’s-t test.