Figure 6. Inhibition of PI3K/Akt signaling aggravates hepatic ischemia-reperfusion injury in mice. (A) Representative H&E staining of liver tissues at 6 h after reperfusion in WT + IR and WT + IR + PX866 groups (×200). (B) Analysis of serum ALT and AST levels (C) and (D), TUNEL positive cells (green) at 6 h after reperfusion. (E) and (F), PCNA protein in liver tissues detected by immunohistochemical staining (×200). (G) and (H), PCNA protein in liver tissue 6 h after reperfusion detected by Western Blotting. The data are presented as the Mean ± SD, n = 6. *P<0.01, compared to Sham group, #P< 0.01, compared to WT + IR group by Student’s-t test.