Research Paper Volume 13, Issue 2 pp 2251—2263

β-Arrestin-2 attenuates hepatic ischemia-reperfusion injury by activating PI3K/Akt signaling

ARRB2 protects hepatocytes against IR injury by activating the PI3K/Akt pathway. L02 cells were treated with H2O2 to mimic oxidative stress during ischemia reperfusion. (A) Overexpress of ARRB2 marked increases in PI3K and Akt phosphorylation and PCNA expression as well as notably decreased cleaved caspase-3 levels. (B) Knockdown of ARRB2 significantly inhibited PI3K/Akt phosphorylation, inhibited PCNA expression and increased cleaved caspase-3 expression. (C) PI3K/Akt inhibitor PX866 significantly inhibited ARRB2 dinduced-PI3K/Akt phosphorylation, downregulated PCNA expression and upregulated cleaved caspase-3 expression. The data are presented as the Mean ± SD, n = 3. PPP

Figure 7. ARRB2 protects hepatocytes against IR injury by activating the PI3K/Akt pathway. L02 cells were treated with H2O2 to mimic oxidative stress during ischemia reperfusion. (A) Overexpress of ARRB2 marked increases in PI3K and Akt phosphorylation and PCNA expression as well as notably decreased cleaved caspase-3 levels. (B) Knockdown of ARRB2 significantly inhibited PI3K/Akt phosphorylation, inhibited PCNA expression and increased cleaved caspase-3 expression. (C) PI3K/Akt inhibitor PX866 significantly inhibited ARRB2 dinduced-PI3K/Akt phosphorylation, downregulated PCNA expression and upregulated cleaved caspase-3 expression. The data are presented as the Mean ± SD, n = 3. P<0.05, P< 0.01, P< 0.001using Student’s-t test.