Research Paper Volume 13, Issue 2 pp 2264—2278

Silencing LINC00482 inhibits tumor-associated inflammation and angiogenesis through down-regulation of MMP-15 via FOXA1 in bladder cancer

Silencing LINC00482 inhibits inflammation and angiogenesis. (A) The silencing efficiency of LINC00482 analysis by RT-qPCR; (B) The tube formation ability after treatment of LINC00482-ASO (× 200); (C) Representative images of Transwell assay and quantification of migratory cells upon treatment of LINC00482-ASO and NC-ASO (× 200); (D) the expression of VEGF and NF-κB detected by Western blot analysis; (E) the levels of TNF-α, IL-1β, IL-6 tested by ELISA. The measurement data were presented as mean ± standard deviations. The differences between two groups were compared by unpaired t test. Experiments were repeated three times; * p vs. cells treated with NC-ASO.

Figure 3. Silencing LINC00482 inhibits inflammation and angiogenesis. (A) The silencing efficiency of LINC00482 analysis by RT-qPCR; (B) The tube formation ability after treatment of LINC00482-ASO (× 200); (C) Representative images of Transwell assay and quantification of migratory cells upon treatment of LINC00482-ASO and NC-ASO (× 200); (D) the expression of VEGF and NF-κB detected by Western blot analysis; (E) the levels of TNF-α, IL-1β, IL-6 tested by ELISA. The measurement data were presented as mean ± standard deviations. The differences between two groups were compared by unpaired t test. Experiments were repeated three times; * p < 0.05 vs. cells treated with NC-ASO.