Research Paper Volume 13, Issue 2 pp 2330—2347

CPEB3 regulates neuron-specific alternative splicing and involves neurogenesis gene expression

Validation of CPEB3 regulated AS events. (A–C) IGV-sashimi plot showed an alternative 3’ splicing sites (A), an exon skipping (B), and a cassette exon (C) events in three different genes. Reads variations on the alternative exon were plotted in the left panel with the transcripts shown below. The schematic diagrams depict the structures of ASEs, AS1 (purple line), and AS2 (green line). The constitutive exon sequences are denoted by black boxes, intron sequences by a horizontal line (right panel, top), while alternative exon by the red box. RNA-seq quantification and RT-qPCR validation of ASEs are shown at the bottom of the right panel. Error bars represent mean ± SEM. *p

Figure 3. Validation of CPEB3 regulated AS events. (AC) IGV-sashimi plot showed an alternative 3’ splicing sites (A), an exon skipping (B), and a cassette exon (C) events in three different genes. Reads variations on the alternative exon were plotted in the left panel with the transcripts shown below. The schematic diagrams depict the structures of ASEs, AS1 (purple line), and AS2 (green line). The constitutive exon sequences are denoted by black boxes, intron sequences by a horizontal line (right panel, top), while alternative exon by the red box. RNA-seq quantification and RT-qPCR validation of ASEs are shown at the bottom of the right panel. Error bars represent mean ± SEM. *p < 0.05.