Research Paper Volume 13, Issue 2 pp 2348—2364

Upregulation of HOTAIRM1 increases migration and invasion by glioblastoma cells

HOTAIRM1 promotes migration and invasion of GBM cells and tumor growth in vivo. (A) Relative expression of HOTAIRM1 in cells transfected with HOTAIRM1 shRNA and negative control. (B) Wound healing assays were used to analyze migration of GBM cells. (C) Matrigel invasion assays were used to analyze invasion of GBM cells. (D) EMT-associated proteins in GBM cells were determined using western blotting. (E) Representative images of subcutaneous tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells on the da\ ys indicated. (F) Growth curve of tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells. (G) Weight of tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells. (H) Representative IHC results of CDH1, N-cadherin and Vimentin in tumors. *P P

Figure 2. HOTAIRM1 promotes migration and invasion of GBM cells and tumor growth in vivo. (A) Relative expression of HOTAIRM1 in cells transfected with HOTAIRM1 shRNA and negative control. (B) Wound healing assays were used to analyze migration of GBM cells. (C) Matrigel invasion assays were used to analyze invasion of GBM cells. (D) EMT-associated proteins in GBM cells were determined using western blotting. (E) Representative images of subcutaneous tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells on the da\ ys indicated. (F) Growth curve of tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells. (G) Weight of tumors originated from sh-ctrl– or sh-HOTAIRM1–transfected pGBM1 cells. (H) Representative IHC results of CDH1, N-cadherin and Vimentin in tumors. *P < 0.05, **P < 0.01.