Figure 5. Estradiol inhibited the proliferation and increased the apoptosis of liver cancer cell lines. (A) Quantification of the apoptotic HCC cells population by flow cytometry. (B) Cell proliferation in HCC cells with different treatments was assessed using Cell-Light EdU Apollo 567 (catalog no· C10310-1; RiboBio). EdU positive cell rates were calculated; scale bar 50μm. Control (DMSO). *p<0.05, **p < 0·01, ***p<0·001. P < 0·05 was considered statistically significant. P values were calculated with Student's t-test.