Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

The expression of lncRNA MIAT in LPS-induced pneumonia. 10 μg LPS was intraperitoneally injected into mice to establish mouse model of pneumonia. (A) H&E staining for mice lung sections. Scale bar, 60 μm. (B) Wet dry mass ratio (W/D) of lungs was calculated. (C) The expression of inflammatory factors IL 1β, IL 6 and TNFα was detected by qRT-PCR. (D) The expression of epithelial markers E-cadherin and Sftpc was determined by qRT-PCR. (E) LncRNA expression profiles in mice with saline or LPS. (F) The expression of MIAT in saline and LPS injected lungs was detected by qRT-PCR. (G) FISH assay was used to determine the location and level of MIAT upon LPS treatment. Scale bar, 100 μm. Data are mean ± SD; *P

Figure 1. The expression of lncRNA MIAT in LPS-induced pneumonia. 10 μg LPS was intraperitoneally injected into mice to establish mouse model of pneumonia. (A) H&E staining for mice lung sections. Scale bar, 60 μm. (B) Wet dry mass ratio (W/D) of lungs was calculated. (C) The expression of inflammatory factors IL 1β, IL 6 and TNFα was detected by qRT-PCR. (D) The expression of epithelial markers E-cadherin and Sftpc was determined by qRT-PCR. (E) LncRNA expression profiles in mice with saline or LPS. (F) The expression of MIAT in saline and LPS injected lungs was detected by qRT-PCR. (G) FISH assay was used to determine the location and level of MIAT upon LPS treatment. Scale bar, 100 μm. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.