Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

Knockdown of MIAT attenuated LPS-induced inflammation and injury in cells. siRNA of MIAT was transfected into TC-1 cells with LPS treatment (100 ng/mL). (A) The silencing efficiency of si-MIAT was detected by qRT-PCR. (B) MTT assay for cell viability of TC-1 cells. (C) Apoptosis cell numbers were tested by TUNLE staining. Scale bar, 100 μm. (D) Western blot for apoptosis related proteins (cleaved-caspase-3, Bax, Bcl2) in TC-1 cells. (E) Flow cytometry assay used to detect early and late apoptosis cell numbers. (F) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (G) Western blot for TNFα and IL 6 expression. (H) Western blot for NF-κB signaling gene IκBα and p65. Data are mean ± SD; *P

Figure 3. Knockdown of MIAT attenuated LPS-induced inflammation and injury in cells. siRNA of MIAT was transfected into TC-1 cells with LPS treatment (100 ng/mL). (A) The silencing efficiency of si-MIAT was detected by qRT-PCR. (B) MTT assay for cell viability of TC-1 cells. (C) Apoptosis cell numbers were tested by TUNLE staining. Scale bar, 100 μm. (D) Western blot for apoptosis related proteins (cleaved-caspase-3, Bax, Bcl2) in TC-1 cells. (E) Flow cytometry assay used to detect early and late apoptosis cell numbers. (F) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (G) Western blot for TNFα and IL 6 expression. (H) Western blot for NF-κB signaling gene IκBα and p65. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.