Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

MIAT acted as a sponge of miR-147a. (A) MiRanda database showing the binding sites of miR-147a with MIAT, and the mutant sequence of MIAT. (B) Wild type and mutant MIAT was transfected into HEK293 cells with or without miR-147a, and luciferase assay was to evaluate the binding between miR-147a and MIAT. (C) TC-1 cells were transfected with MIAT plasmid or si-MIAT or its NC, the mRNA level of miR-147a was detected using qRT-PCR. (D) Biotinylated miR-147a or NC was transfected into H460 cells, and qRT-PCR was performed to detect the enrichment of MIAT. Data are mean ± SD; *P

Figure 4. MIAT acted as a sponge of miR-147a. (A) MiRanda database showing the binding sites of miR-147a with MIAT, and the mutant sequence of MIAT. (B) Wild type and mutant MIAT was transfected into HEK293 cells with or without miR-147a, and luciferase assay was to evaluate the binding between miR-147a and MIAT. (C) TC-1 cells were transfected with MIAT plasmid or si-MIAT or its NC, the mRNA level of miR-147a was detected using qRT-PCR. (D) Biotinylated miR-147a or NC was transfected into H460 cells, and qRT-PCR was performed to detect the enrichment of MIAT. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.