Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

NKAP was a directed target of miR-147a. (A) The binding bases of miR-147a and NKAP from Targetscan. (B) Wild type and mutant NKAP was transfected into HEK293 cells with or without miR-147a, and luciferase assay was to evaluate the binding. TC-1 cells were transfected with miR-147a or AMO-147a, (C) the mRNA level of NKAP was detected. (D) RIP assay for the binding of miR-147a and NKAP in H460 cells. Data are mean ± SD; *P

Figure 5. NKAP was a directed target of miR-147a. (A) The binding bases of miR-147a and NKAP from Targetscan. (B) Wild type and mutant NKAP was transfected into HEK293 cells with or without miR-147a, and luciferase assay was to evaluate the binding. TC-1 cells were transfected with miR-147a or AMO-147a, (C) the mRNA level of NKAP was detected. (D) RIP assay for the binding of miR-147a and NKAP in H460 cells. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.