Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

Deletion of MIAT alleviates LPS-induced inflammation and injury via regulating miR-147a in lung tissues. LV-sh-MIAT was intratracheally injected into mice LV-sh-MIAT or LV-sh-NC was intratracheally injected into mice. (A) The efficiency of sh-MIAT and AMO-147a was determined by qRT-PCR. (B) Survival plots for mice in different groups. (C) Wet dry mass ratio (W/D) of lungs was calculated. (D) Myeloperoxidase (MPO) of lungs was examined. (E) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (F) qRT-PCR analysis for E-cadherin and Sftpc expression. Data are mean ± SD; *P

Figure 7. Deletion of MIAT alleviates LPS-induced inflammation and injury via regulating miR-147a in lung tissues. LV-sh-MIAT was intratracheally injected into mice LV-sh-MIAT or LV-sh-NC was intratracheally injected into mice. (A) The efficiency of sh-MIAT and AMO-147a was determined by qRT-PCR. (B) Survival plots for mice in different groups. (C) Wet dry mass ratio (W/D) of lungs was calculated. (D) Myeloperoxidase (MPO) of lungs was examined. (E) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (F) qRT-PCR analysis for E-cadherin and Sftpc expression. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.