Figure 3. LOC389641 cellular location and knockdown impairs cell proliferation. (A–C) qRT-PCR showing the nuclear and cytoplasmic fractions of LOC389641 in H1299, H838 and PC-9 cells. GAPDH is used as cytoplasmic control and U1 snRNA as nuclear control. LOC389641 is primarily in cytoplasm (61% - 77%). (D) LOC389641 siRNA knockdown efficiency (48 h) in H1299, H838 and PC-9 cell lines measured by qRT-PCR. GAPDH is used as loading control. (E) Cell proliferation is decreased after LOC389641 siRNAs treatment in H1299, H838 and PC-9 cell lines. ** p < 0.01 by t test. (F, G) Flow cytometry analysis to evaluate the effects of LOC389641 on cell cycle distribution in H1299 and H838 cell lines.