Research Paper Volume 13, Issue 5 pp 6565—6591

Phenotyping of immune and endometrial epithelial cells in endometrial carcinomas revealed by single-cell RNA sequencing

Lymphoid cell clusters in paratumors and endometrial tumors. (A, B) t-SNE plot of 3,223 lymphoid cells in Paratumor (A) and 3,712 lymphoid cells in Tumor (B), color-coded according to their associated cluster (left) or the assigned subtype (right). (C, D) t-SNE plot, color-coded to show the relative expression (gray to red) of marker genes for the lymphoid subtypes in Paratumor (C) and Tumor (D). (E) Heatmap created using known gene expression profiles of lymphoid cells in Paratumor (left panel) and Tumor (right panel). The gene expression profiles include marker genes for cell type and naive, costimulatory, regulatory, exhaustion and cytotoxicity expression signatures. The identity of each cluster was assigned with known markers. (F) The overall survival curves based on TCGA-UCEC data (n = 549 patients), stratified for the average expression (binary: high versus low) of tumor lymphoid cell marker genes.

Figure 5. Lymphoid cell clusters in paratumors and endometrial tumors. (A, B) t-SNE plot of 3,223 lymphoid cells in Paratumor (A) and 3,712 lymphoid cells in Tumor (B), color-coded according to their associated cluster (left) or the assigned subtype (right). (C, D) t-SNE plot, color-coded to show the relative expression (gray to red) of marker genes for the lymphoid subtypes in Paratumor (C) and Tumor (D). (E) Heatmap created using known gene expression profiles of lymphoid cells in Paratumor (left panel) and Tumor (right panel). The gene expression profiles include marker genes for cell type and naive, costimulatory, regulatory, exhaustion and cytotoxicity expression signatures. The identity of each cluster was assigned with known markers. (F) The overall survival curves based on TCGA-UCEC data (n = 549 patients), stratified for the average expression (binary: high versus low) of tumor lymphoid cell marker genes.