Research Paper Volume 13, Issue 5 pp 6565—6591

Phenotyping of immune and endometrial epithelial cells in endometrial carcinomas revealed by single-cell RNA sequencing

Summary map of the endometrial carcinoma ecosystem. The endometrium is the inner lining of the uterus and consists of epithelial and stromal cells. These are further divided into glandular (GE) and luminal (LE) epithelium. The basalis layer is the source for the regeneration of the endometrium. The two major zones of the endometrium, the basalis and the functionalis, are functionally divided while not anatomically partitioned. The subtype of epithelial cells was not confirmed by immunohistochemical staining and is inferred as a result of pathway analysis and transcriptional similarity to the published gene expression patterns. Stromal cells consist of endothelial cells, fibroblasts, myeloid cells- including DCs, monocytes, macrophages, mast cells, and lymphocytes- including B cells, T cells, ILC3s and NK cells. Exhausted CD8+ T cells and macrophages are preferentially enriched in tumor. CD8+ T cells and macrophages show continuous activation pattern among distinct cell subsets.

Figure 9. Summary map of the endometrial carcinoma ecosystem. The endometrium is the inner lining of the uterus and consists of epithelial and stromal cells. These are further divided into glandular (GE) and luminal (LE) epithelium. The basalis layer is the source for the regeneration of the endometrium. The two major zones of the endometrium, the basalis and the functionalis, are functionally divided while not anatomically partitioned. The subtype of epithelial cells was not confirmed by immunohistochemical staining and is inferred as a result of pathway analysis and transcriptional similarity to the published gene expression patterns. Stromal cells consist of endothelial cells, fibroblasts, myeloid cells- including DCs, monocytes, macrophages, mast cells, and lymphocytes- including B cells, T cells, ILC3s and NK cells. Exhausted CD8+ T cells and macrophages are preferentially enriched in tumor. CD8+ T cells and macrophages show continuous activation pattern among distinct cell subsets.