Research Paper Volume 13, Issue 2 pp 2575—2592

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial–mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway

Different radiosensitivity, motility and EMT potential in radioresistant and paternal cells. (A) Count of cell colonies formed 10-12 days after 2-10 Gy radiation treatment. (B) Cell migration analyzed by wound healing assay using wound gap photographs for A549-RR, A549-control, H1299-RR and H1299-control cells. **P C) A549-RR, A549-control, H1299-RR and H1299-control cells were subjected to Matrigel invasion assay and photographed (magnification 20×, scale bar 100 μm) for the analysis of their invasion capacity. **P D) Representative 3D-invasion images of cells. Scale bar:100 μm. (E) Protein levels of E-Cadherin, N-Cadherin and Vimentin measured by Western blot. GAPDH served as the loading control. (F) Vimentin (red) immunofluorescence images of cells. Nuclei were stained with PI (blue) All data from the RR cell line were collected between 5 and 6 weeks post-radiation treatment. All results were from three independent experiments and are presented as mean ± SD. P-values were calculated by student’s t-test.

Figure 1. Different radiosensitivity, motility and EMT potential in radioresistant and paternal cells. (A) Count of cell colonies formed 10-12 days after 2-10 Gy radiation treatment. (B) Cell migration analyzed by wound healing assay using wound gap photographs for A549-RR, A549-control, H1299-RR and H1299-control cells. **P < 0.01, significant difference between radioresistance and control cells. (C) A549-RR, A549-control, H1299-RR and H1299-control cells were subjected to Matrigel invasion assay and photographed (magnification 20×, scale bar 100 μm) for the analysis of their invasion capacity. **P < 0.01, significant difference between A549-RR and A549-control cells. (D) Representative 3D-invasion images of cells. Scale bar:100 μm. (E) Protein levels of E-Cadherin, N-Cadherin and Vimentin measured by Western blot. GAPDH served as the loading control. (F) Vimentin (red) immunofluorescence images of cells. Nuclei were stained with PI (blue) All data from the RR cell line were collected between 5 and 6 weeks post-radiation treatment. All results were from three independent experiments and are presented as mean ± SD. P-values were calculated by student’s t-test.