Figure 4. Effect of β-elemene and RT on the expression of EMT/CSC markers and radiosensitivity in A549 cells. A549 cells were treated with single RT (4 Gy) for 12 h or β-elemene 12 h prior to RT (4 Gy). (A) Representative images of colony formation for the different treatments. (B) After corresponding treatment, the cells were subjected to Matrigel invasion assay and photographed. Invasion capability of cells was calculation. (C) Representative 3D-invasion images of cells. Scale bar:100 μm. (D) Protein levels of Vimentin, E-Cadherin and N-Cadherin were measured by Western blot. (E) Immunofluorescence images of Vimentin (red) Nuclei were stained with PI (blue). (F) Representative images of spheroid formation after the different treatments. (G) The diameter and number of tumor cell spheres in (E). (H) Protein levels of CD133, CD44, and Epcam measured by Western blot.