Research Paper Volume 13, Issue 2 pp 2575—2592

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial–mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway

Effect of β-elemene and RT on cell cycle and DNA damage repair in A549 cells. A549 cells were treated with single RT (4 Gy) or β-elemene prior to RT (4 Gy). (A) After each treatment, cell cycle distribution was analyzed with flow cytometry. (B) The percentage of cells at each phase of the cell cycle was quantified. (C) A549 cells were harvested 48 h after different treatments and subjected to the comet assay, as described. (D) The percentage of DNA in the tail was calculated for 50 random cells. (E) DSB marker (γH2AX), and homologous recombination pathway-related proteins (RAD51) were measured by Western blot. Representative images from three independent experiments are shown.

Figure 5. Effect of β-elemene and RT on cell cycle and DNA damage repair in A549 cells. A549 cells were treated with single RT (4 Gy) or β-elemene prior to RT (4 Gy). (A) After each treatment, cell cycle distribution was analyzed with flow cytometry. (B) The percentage of cells at each phase of the cell cycle was quantified. (C) A549 cells were harvested 48 h after different treatments and subjected to the comet assay, as described. (D) The percentage of DNA in the tail was calculated for 50 random cells. (E) DSB marker (γH2AX), and homologous recombination pathway-related proteins (RAD51) were measured by Western blot. Representative images from three independent experiments are shown.